Mapping of antigenic sites on the nucleocapsid protein of the severe acute respiratory syndrome coronavirus
Identifieur interne : 005B10 ( Main/Exploration ); précédent : 005B09; suivant : 005B11Mapping of antigenic sites on the nucleocapsid protein of the severe acute respiratory syndrome coronavirus
Auteurs : YUXIAN HE [États-Unis] ; YUSEN ZHOU [République populaire de Chine] ; HAO WU [République populaire de Chine] ; ZHIHUA KOU [République populaire de Chine] ; SHUWEN LIU [États-Unis] ; SHIBO JIANG [États-Unis]Source :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2004.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
Antigenic sites on the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) were mapped by Pepscan analysis with overlapping peptides that span the N protein sequence. Two major immunodominant epitopes located in the C-terminal region (amino acids [aal 362 to 412) and middle region (aa 153 to 178) reacted with more than 75% of sera from SARS patients. Several minor immunodominant epitopes were reactive with about 50% of the SARS sera. Antisera from mice immunized with inactivated SARS-CoV recognized the two major immunodominant epitopes and one antigenic site located adjacent to the N-terminal region (aa 76 to 101), which did not react with the sera from SARS patients. Several monoclonal antibodies against SARS-CoV bound to the N- or C-terminal antigenic sites. These results suggest that the above antigenic sites on the N protein are important in eliciting humoral immune response against SARS-CoV in humans and animals and can be used as antigens for developing diagnostic tests.
Affiliations:
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Le document en format XML
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Kimball Research Institute, New York Blood Center</s1><s2>New York, New York</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Yusen Zhou" sort="Yusen Zhou" uniqKey="Yusen Zhou" last="Yusen Zhou">YUSEN ZHOU</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Beijing Institute of Microbiology and Epidemiology, Capital University of Medical Sciences</s1><s2>Beijing</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Hao Wu" sort="Hao Wu" uniqKey="Hao Wu" last="Hao Wu">HAO WU</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>YouAn Hospital, Capital University of Medical Sciences</s1><s2>Beijing</s2><s3>CHN</s3><sZ>3 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhihua Kou" sort="Zhihua Kou" uniqKey="Zhihua Kou" last="Zhihua Kou">ZHIHUA KOU</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Beijing Institute of Microbiology and Epidemiology, Capital University of Medical Sciences</s1><s2>Beijing</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Shuwen Liu" sort="Shuwen Liu" uniqKey="Shuwen Liu" last="Shuwen Liu">SHUWEN LIU</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Lindsley F. Kimball Research Institute, New York Blood Center</s1><s2>New York, New York</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Shibo Jiang" sort="Shibo Jiang" uniqKey="Shibo Jiang" last="Shibo Jiang">SHIBO JIANG</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Lindsley F. Kimball Research Institute, New York Blood Center</s1><s2>New York, New York</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of clinical microbiology : (Print)</title><title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title><idno type="ISSN">0095-1137</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of clinical microbiology : (Print)</title><title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title><idno type="ISSN">0095-1137</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term><term>Microbiology</term><term>Nucleocapsid</term><term>Protein</term><term>Severe acute respiratory syndrome</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Nucléocapside</term><term>Protéine</term><term>Microbiologie</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Antigenic sites on the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) were mapped by Pepscan analysis with overlapping peptides that span the N protein sequence. Two major immunodominant epitopes located in the C-terminal region (amino acids [aal 362 to 412) and middle region (aa 153 to 178) reacted with more than 75% of sera from SARS patients. Several minor immunodominant epitopes were reactive with about 50% of the SARS sera. Antisera from mice immunized with inactivated SARS-CoV recognized the two major immunodominant epitopes and one antigenic site located adjacent to the N-terminal region (aa 76 to 101), which did not react with the sera from SARS patients. Several monoclonal antibodies against SARS-CoV bound to the N- or C-terminal antigenic sites. These results suggest that the above antigenic sites on the N protein are important in eliciting humoral immune response against SARS-CoV in humans and animals and can be used as antigens for developing diagnostic tests.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>Pékin</li></settlement></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Yuxian He" sort="Yuxian He" uniqKey="Yuxian He" last="Yuxian He">YUXIAN HE</name></region><name sortKey="Shibo Jiang" sort="Shibo Jiang" uniqKey="Shibo Jiang" last="Shibo Jiang">SHIBO JIANG</name><name sortKey="Shuwen Liu" sort="Shuwen Liu" uniqKey="Shuwen Liu" last="Shuwen Liu">SHUWEN LIU</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Yusen Zhou" sort="Yusen Zhou" uniqKey="Yusen Zhou" last="Yusen Zhou">YUSEN ZHOU</name></noRegion><name sortKey="Hao Wu" sort="Hao Wu" uniqKey="Hao Wu" last="Hao Wu">HAO WU</name><name sortKey="Zhihua Kou" sort="Zhihua Kou" uniqKey="Zhihua Kou" last="Zhihua Kou">ZHIHUA KOU</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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